All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Monitoring of circulating tumor cells by a combination of immunomagnetic enrichment and RT-PCR in colorectal cancer patients undergoing surgery

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F16%3AN0000081" target="_blank" >RIV/00064190:_____/16:N0000081 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11110/16:10333687

  • Result on the web

    <a href="http://dx.doi.org/10.17219/acem/63824" target="_blank" >http://dx.doi.org/10.17219/acem/63824</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17219/acem/63824" target="_blank" >10.17219/acem/63824</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Monitoring of circulating tumor cells by a combination of immunomagnetic enrichment and RT-PCR in colorectal cancer patients undergoing surgery

  • Original language description

    The presence of circulating tumor cells (CTC) has been reported in patients with advanced colorectal cancer. Monitoring CTC (also known as a liquid-biopsy) has recently become the center of interest for low-invasive monitoring of cancer progression and predictive biomarkers testing. Along with high-cost technology and a complex methodology, a straightforward method based on magnetic beads enrichment followed by RT-PCR is set to allow for routine CTC analysis in colorectal cancer patients. Objectives. The main purpose of this study was to evaluate the possibility of CTC detection in routine monitoring of patients starting before and continuing after surgery. Material and Methods. The investigated group consisted of 30 patients mainly in advanced stages of colorectal cancer. In all patients, CTC detection was performed prior to surgery, in a subset of 14 patients additional sampling was done during and after surgery. In all cases, peripheral blood was processed using AdnaTest ColonCancer kit, which relies on enriching CTCs using EpCAM-functionalized magnetic beads and subsequently identifying tumorspecific CEA, EGFR and GA733-2 mRNA transcripts. Results. Out of all the tested samples, CTC were found in one patient suffering from advanced disease with lung and liver metastases. There, however, the positive finding was confirmed in 3 consecutive samples acquired before, during and shortly after palliative R2 resection. Conclusions. The presence of CTC may be used to observe post-operative disease development. Due to the overall low CTC detection, further technology development may be necessary before its universal applicability to manage colorectal cancer patients.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FJ - Surgery including transplantology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Advances in Clinical and Experimental Medicine

  • ISSN

    1899-5276

  • e-ISSN

  • Volume of the periodical

    25

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    PL - POLAND

  • Number of pages

    6

  • Pages from-to

    1273-1279

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85006837017