Monitoring of circulating tumor cells by a combination of immunomagnetic enrichment and RT-PCR in colorectal cancer patients undergoing surgery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F16%3AN0000081" target="_blank" >RIV/00064190:_____/16:N0000081 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10333687

Result on the web

<a href="http://dx.doi.org/10.17219/acem/63824" target="_blank" >http://dx.doi.org/10.17219/acem/63824</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17219/acem/63824" target="_blank" >10.17219/acem/63824</a>

Result language

angličtina

Original language name

Monitoring of circulating tumor cells by a combination of immunomagnetic enrichment and RT-PCR in colorectal cancer patients undergoing surgery

Original language description

The presence of circulating tumor cells (CTC) has been reported in patients with advanced colorectal cancer. Monitoring CTC (also known as a liquid-biopsy) has recently become the center of interest for low-invasive monitoring of cancer progression and predictive biomarkers testing. Along with high-cost technology and a complex methodology, a straightforward method based on magnetic beads enrichment followed by RT-PCR is set to allow for routine CTC analysis in colorectal cancer patients. Objectives. The main purpose of this study was to evaluate the possibility of CTC detection in routine monitoring of patients starting before and continuing after surgery. Material and Methods. The investigated group consisted of 30 patients mainly in advanced stages of colorectal cancer. In all patients, CTC detection was performed prior to surgery, in a subset of 14 patients additional sampling was done during and after surgery. In all cases, peripheral blood was processed using AdnaTest ColonCancer kit, which relies on enriching CTCs using EpCAM-functionalized magnetic beads and subsequently identifying tumorspecific CEA, EGFR and GA733-2 mRNA transcripts. Results. Out of all the tested samples, CTC were found in one patient suffering from advanced disease with lung and liver metastases. There, however, the positive finding was confirmed in 3 consecutive samples acquired before, during and shortly after palliative R2 resection. Conclusions. The presence of CTC may be used to observe post-operative disease development. Due to the overall low CTC detection, further technology development may be necessary before its universal applicability to manage colorectal cancer patients.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FJ - Surgery including transplantology

OECD FORD branch

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Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advances in Clinical and Experimental Medicine

ISSN

1899-5276

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

6

Country of publishing house

PL - POLAND

Number of pages

6

Pages from-to

1273-1279

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85006837017