Neutrophil phenotypes in bronchial airways differentiate single from dual responding allergic asthmatics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F23%3A10001020" target="_blank" >RIV/00064190:_____/23:10001020 - isvavai.cz</a>
Alternative codes found
RIV/00064190:_____/22:N0000085 RIV/00216208:11110/23:10444903
Result on the web
<a href="https://doi.org/10.1111/cea.14149" target="_blank" >https://doi.org/10.1111/cea.14149</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/cea.14149" target="_blank" >10.1111/cea.14149</a>
Alternative languages
Result language
angličtina
Original language name
Neutrophil phenotypes in bronchial airways differentiate single from dual responding allergic asthmatics
Original language description
Introduction: Allergic asthmatics with both an early (EAR) and a late allergic reaction (LAR) following allergen exposure are termed 'dual responders' (DR), while 'single responders' (SR) only have an EAR. Mechanisms that differentiate DR from SR are largely unknown, particularly regarding the role and phenotypes of neutrophils. Therefore, we aimed to study neutrophils in DR and SR asthmatics. Methods: Thirty-four allergic asthmatics underwent an inhaled allergen challenge, samples were collected before and up to 24 h post-challenge. Cell differentials were counted from bronchial lavage, alveolar lavage and blood; and tissue neutrophils were quantified in immune-stained bronchial biopsies. Lavage neutrophil nuclei lobe segmentation was used to classify active (1-4 lobes) from suppressive neutrophils (GREATER-THAN OR EQUAL TO5 lobes). Levels of transmigration markers: soluble (s)CD62L and interleukin-1Ra, and activity markers: neutrophil elastase (NE), DNA-histone complex and dsDNA were measured in lavage fluid and plasma. Results: Compared with SR at baseline, DR had more neutrophils in their bronchial airways at baseline, both in the lavage (p =.0031) and biopsies (p =.026) and elevated bronchial neutrophils correlated with less antitransmigratory IL-1Ra levels (r = MINUS SIGN 0.64). DR airways had less suppressive neutrophils and more 3-lobed (active) neutrophils (p =.029) that correlated with more bronchial lavage histone (p =.020) and more plasma NE (p =.0016). Post-challenge, DR released neutrophil extracellular trap factors in the blood earlier and had less pro-transmigratory sCD62L during the late phase (p =.0076) than in SR. Conclusion: DR have a more active airway neutrophil phenotype at baseline and a distinct neutrophil response to allergen challenge that may contribute to the development of an LAR. Therefore, neutrophil activity should be considered during targeted diagnosis and bio-therapeutic development for DR. (C) 2022 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30225 - Allergy
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical and Experimental Allergy
ISSN
0954-7894
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
65-77
UT code for WoS article
000794190600001
EID of the result in the Scopus database
2-s2.0-85128966981