Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous I3-catenin and cytoplasmic I3-catenin expression with the outcome of patients with clear cell renal cell carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F25%3A10001103" target="_blank" >RIV/00064190:_____/25:10001103 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/26:00564637 RIV/00216224:14110/25:00140234

Result on the web

<a href="https://biomed.papers.upol.cz/corproof.php?tartkey=bio-000000-3573" target="_blank" >https://biomed.papers.upol.cz/corproof.php?tartkey=bio-000000-3573</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2024.005" target="_blank" >10.5507/bp.2024.005</a>

Result language

angličtina

Original language name

Association of the combined parameters including the frequency of primary cilia, PD-L1, Smoothened protein, membranous I3-catenin and cytoplasmic I3-catenin expression with the outcome of patients with clear cell renal cell carcinoma

Original language description

Aims. The objective of this study was to investigate the association and combined prognostic significance of the PD -L1, Smoothened protein and beta-catenin expressions in patients with clear cell renal cell carcinoma (ccRCC). Methods.The PD -L1, Smoothened protein and beta-catenin expression were evaluated in 104 ccRCC patients. All studied tumor samples were acquired from nephrectomy specimens of primary tumors and not from biopsies or metastases. An indirect immunohistochemistry using polyclonal rabbit anti -Smoothened antibody, monoclonal mouse anti -human beta-catenin-1 antibody, immunohistochemical assay PD -L1 28-8 pharmDx using monoclonal rabbit anti -PD -L1 antibody and anti-VHL (C- terminal) rabbit antibody was used. Immunohistochemistry was scored semiquantitavely. Results. Median overall survival (OS) was significantly better in patients with lower PD -L1 expression (&lt;= 5%), Smoothened protein (SMO) expression (&lt;5%) or cytoplasmic beta-catenin expression (&lt;= 75%) than in patients with higher expressions of these biomarkers (P&lt;0.001, P=0.047, and P&lt;0.001, respectively). Membranous beta-catenin showed an opposite effect with its lower expression (&lt;= 75%) being associated with longer OS (P=0.020). There was significant association between PD -1 and PD -L1 expression (P=0.007) and significant association of tumor grade (WHO 2016) with membranous beta-catenin (P&lt;0.001), cytoplasmic beta-catenin (P=0.005), pVHL (P=0.042), PD -L1 (P=0.049) and PD -1 (P=0.028) expression. Conclusion. The present study provides the first data on the potential association and combined prognostic significance of frequency of primary cilia, PD -L1, Smoothened protein and beta-catenin expression with the outcome in clear cell renal cell carcinoma.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

<a href="/en/project/LX22NPO5102" target="_blank" >LX22NPO5102: National institute for cancer research</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1213-8118

e-ISSN

1804-7521

Volume of the periodical

169

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

107-115

UT code for WoS article

001179455000001

EID of the result in the Scopus database

2-s2.0-105008374155