Genotyping and transmission analysis of Mycobacterium tuberculosis in a pediatric population in Czech Republic and Slovakia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F25%3A10001383" target="_blank" >RIV/00064190:_____/25:10001383 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1186/s12879-025-11284-9" target="_blank" >https://doi.org/10.1186/s12879-025-11284-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12879-025-11284-9" target="_blank" >10.1186/s12879-025-11284-9</a>

Result language

angličtina

Original language name

Genotyping and transmission analysis of Mycobacterium tuberculosis in a pediatric population in Czech Republic and Slovakia

Original language description

BackgroundTuberculosis remains a global health concern, with rising pediatric and adolescent cases. The advancement of diagnostic strategies is crucial for effective control, with whole-genome sequencing emerging as a promising tool. This study explores using whole-genome sequencing in pediatric Tuberculosis.MethodsMycobacterium tuberculosis isolates from pediatric patients and their contacts were collected between January 2023 and June 2024 in Slovakia and the Czech Republic. The isolates were subjected to WGS to characterize the resistance patterns and transmission.ResultsThe study included 37 patients in total-30 pediatric cases and 7 adult index cases-with a single M. tuberculosis isolate collected per patient. The phylogenetic analysis results revealed that 32 out of 37 (86.5%) isolates belonged to the Euro-American lineage. Five isolates (13.5%) belonged to the East-Asian lineage. Genotypic resistance to at least one drug was confirmed in 6 patients (16%). 24 patients were divided into 9 clusters (65%), leaving 13 unclustered (35%). Moreover, the concordance between the identification of source case by WGS and epidemiological anamnesis was confirmed in 60% of patients.ConclusionsEpidemiological data may not always provide accurate insights into the transmission of TB. Consequently, integrating molecular methods, such as WGS, is essential to enhance the reliability and precision of epidemiological analyses.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30303 - Infectious Diseases

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC INFECTIOUS DISEASES

ISSN

—

e-ISSN

1471-2334

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

nestránkováno

UT code for WoS article

001522894500001

EID of the result in the Scopus database

2-s2.0-105010148591