Use of colony-based bacterial strain typing for tracking the fate of Lactobacillus strains during human consumption

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F09%3A5415" target="_blank" >RIV/00064203:_____/09:5415 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/09:5415

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Use of colony-based bacterial strain typing for tracking the fate of Lactobacillus strains during human consumption

Original language description

We developed a RAPD typing scheme to genetically type Lactic Acid Bacteria, and optimised the method for direct application to bacterial colony growth. A high-throughput strategy for fingerprinting the cultivable diversity of human faeces was developed and used to determine: (i) the initial cultivable LAB strain diversity in the human gut, and (ii) the fate of two Lactobacillus strains contained within a capsule that was administered in a small-scale human feeding study. We have shown that genetic strain typing of the cultivable human gut microbiota can be evaluated using a high throughput RAPD technique based on single bacterial colonies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Microbiology

ISSN

1471-2180

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

251

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

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UT code for WoS article

000273007900001

EID of the result in the Scopus database

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