Zeptomole Electrochemical Detection of Metallothioneins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6272" target="_blank" >RIV/00064203:_____/10:6272 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/10:00040561 RIV/62156489:43210/10:00166293 RIV/00216208:11130/10:6272

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Zeptomole Electrochemical Detection of Metallothioneins

Original language description

We used adsorptive transfer stripping technique coupled with CPSA for detection of cysteine, glutathione oxidized and reduced, phytochelatin, bovine serum albumin, and metallothionein (MT). We estimated detection limits down to tens of fg per ml. This method was applied to detect metallothioneins in serum from patients with breast cancer and in neuroblastoma cells resistant and sensitive to cisplatin. It was found that MT level in serum was almost twice higher as the level in healthy individuals. This paper brings results on the application of ultra-sensitive electroanalytical method for MT detection. It is worth mentioning that detection of other biologically important molecules is possible by this method. Coupling of this method with isolation by antibody-modified paramagnetic particles may be implemented to lab-on-chip instrument.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/IAA401990701" target="_blank" >IAA401990701: Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

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UT code for WoS article

000279522500009

EID of the result in the Scopus database

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