Low expression of asparagine synthetase in lymphoid blasts precludes its role in sensitivity to L-asparaginase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F12%3A8239" target="_blank" >RIV/00064203:_____/12:8239 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/12:8239

Result on the web

<a href="http://dx.doi.org/10.1016/j.exphem.2012.04.005" target="_blank" >http://dx.doi.org/10.1016/j.exphem.2012.04.005</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Low expression of asparagine synthetase in lymphoid blasts precludes its role in sensitivity to L-asparaginase

Original language description

Childhood acute lymphoblastic leukemia (ALL) is treated with combined chemotherapy, including L-asparaginase (L-asp). Recent studies question the traditional view that the level of asparagine synthetase (ASNS), an enzyme producing the intracellular asparagine, correlates with the response to L-asp treatment. However, the importance of ASNS in response to L-asp has neither been confirmed nor refuted so far. In this study, we wanted to elucidate the effect of ASNS expression level on the sensitivity of ALL cells to L-asp treatment. We used four ALL cell lines (NALM-6, RS4;11, REH, and UOCB6) and 30 diagnostic bone marrow samples of ALL patients to study the relationship between ASNS expression and sensitivity to L-asp using MTS proliferation assay. RNA interference was used to study the effect of a range of ASNS levels on the response to L-asp treatment. Using a cell line model with a gradually knocked-down ASNS gene, we defined a cutoff level below which ASNS gene expression does not co

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NT12429" target="_blank" >NT12429: Mechanism of sensitivity and resistance to L-Asparaginase in leukemic cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental Hematology

ISSN

0301-472X

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

657-665

UT code for WoS article

000307319500006

EID of the result in the Scopus database

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