Development of High-Sensitive ELISA Method for Detection of Adipophilin Levels in Human Colostrum and Breast Milk
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F14%3A10292991" target="_blank" >RIV/00064203:_____/14:10292991 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/14:10292991 RIV/62157124:16370/14:43872867
Result on the web
<a href="http://dx.doi.org/10.1002/jcla.21675" target="_blank" >http://dx.doi.org/10.1002/jcla.21675</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcla.21675" target="_blank" >10.1002/jcla.21675</a>
Alternative languages
Result language
angličtina
Original language name
Development of High-Sensitive ELISA Method for Detection of Adipophilin Levels in Human Colostrum and Breast Milk
Original language description
Aim: To develop and validate high-sensitive (hs) ELISA method for detection of adipophilin (adipose differentiation-related protein, ADRP) in human breast milk (BM) and to analyze adipophilin levels in BM during 12 months of lactation. Methods: ADRP levels were determined using hsELISA method (Biovendor-Laboratory Medicine, Inc.) in colostrum (D0) and BM of 72 mothers was collected 1, 3, 6, and 12 months following delivery (M1, 3, 6, 12). Results: ADRP was detectable in BM up to 12 months of lactation.Mean levels at D0 were 1.98 +/- 0.12; M1, 2.83 +/- 0.21; M3, 2.39 +/- 0.17; M6, 2.57 +/- 0.16; and at M12 3.25 +/- 0.21 g/ml. Significantly higher levels of ADRP were found in M1 and M12 when compared to D0 and in M12 when compared to M3 (overall P = 0.0001). No significant correlation was seen between ADRP levels in BM and adiponectin, body weight of infants, their birth length, body weight gain during the first year of life, or BMI of mothers before pregnancy. Conclusions: We developed
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FG - Paediatrics
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
255-260
UT code for WoS article
000339551900001
EID of the result in the Scopus database
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