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Human interleukin-23 receptor antagonists derived from an albumin-binding domain scaffold inhibit IL-23-dependent ex vivo expansion of IL-17-producing T-cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F14%3A10293045" target="_blank" >RIV/00064203:_____/14:10293045 - isvavai.cz</a>

  • Alternative codes found

    RIV/86652036:_____/14:00431238 RIV/61388971:_____/14:00431238 RIV/67985882:_____/14:00431238 RIV/00216208:11130/14:10293045

  • Result on the web

    <a href="http://dx.doi.org/10.1002/prot.24472" target="_blank" >http://dx.doi.org/10.1002/prot.24472</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/prot.24472" target="_blank" >10.1002/prot.24472</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Human interleukin-23 receptor antagonists derived from an albumin-binding domain scaffold inhibit IL-23-dependent ex vivo expansion of IL-17-producing T-cells

  • Original language description

    Engineered combinatorial libraries derived from small protein scaffolds represent a powerful tool for generating novel binders with high affinity, required specificity and designed inhibitory function. This work was aimed to generate a collection of recombinant binders of human interleukin-23 receptor (IL-23R), which is a key element of proinflammatory IL-23-mediated signaling. A library of variants derived from the three-helix bundle scaffold of the albumin-binding domain (ABD) of streptococcal proteinG and ribosome display were used to select for high-affinity binders of recombinant extracellular IL-23R. A collection of 34 IL-23R-binding proteins (called REX binders), corresponding to 18 different sequence variants, was used to identify a group of ligands that inhibited binding of the recombinant p19 subunit of IL-23, or the biologically active human IL-23 cytokine, to the recombinant IL-23R or soluble IL-23R-IgG chimera. The strongest competitors for IL-23R binding in ELISA were co

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FD - Oncology and haematology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Proteins: Structure, Function and Genetics

  • ISSN

    0887-3585

  • e-ISSN

  • Volume of the periodical

    82

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    975-989

  • UT code for WoS article

    000335955300009

  • EID of the result in the Scopus database