Evaluation of TD test for analysis of persistence or tolerance in clinical isolates of Staphylococcus aureus

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F19%3A10400180" target="_blank" >RIV/00064203:_____/19:10400180 - isvavai.cz</a>
Alternative codes found
RIV/27283933:_____/19:00007538 RIV/00216208:11130/19:10400180 RIV/00216208:11310/19:10400180
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bFydSOQtLt" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bFydSOQtLt</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2019.105705" target="_blank" >10.1016/j.mimet.2019.105705</a>

Result language
angličtina
Original language name
Evaluation of TD test for analysis of persistence or tolerance in clinical isolates of Staphylococcus aureus
Original language description
Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance. The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et al., 2017) to detect persistent or tolerant bacterial cells in clinical isolates of Staphylococcus aureus. The principle of the test is to resuscitate the subpopulation of persistent or tolerant bacterial cells following a disk diffusion test by glucose. Results of the TDtest were evaluated using time killing experiments for three pairs of consecutive S. aureus isolates from lower respiratory airway samples of three cystic fibrosis patients with chronic staphylococcal infections. TDtest enabled semi-quantitative detection of persistent or tolerant bacterial populations in all analyzed isolates for oxacillin, vancomycin, and ciprofloxacin to which isolates studied were susceptible. Therefore, TDtest is a promising method for rapidly determining persistence/tolerance in clinical isolates of S. aureus. (C) 2019 Elsevier B.V.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/NV17-30460A" target="_blank" >NV17-30460A: Molecular characterization of adaptive antibiotic resistance and tolerance mechanisms of Staphylococcus aureus strains causing chronic infections</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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