Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F21%3A10426470" target="_blank" >RIV/00064203:_____/21:10426470 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/21:10426470

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BZfLuiFvWg" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=BZfLuiFvWg</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1136/thoraxjnl-2020-216368" target="_blank" >10.1136/thoraxjnl-2020-216368</a>

Result language

angličtina

Original language name

Rectal organoid morphology analysis (ROMA) as a promising diagnostic tool in cystic fibrosis

Original language description

Diagnosing cystic fibrosis (CF) when sweat chloride is not in the CF range and less than 2 disease-causing CFTR mutations are found requires physiological CFTR assays, which are not always feasible or available. We developed a new physiological CFTR assay based on the morphological differences between rectal organoids from subjects with and without CF. In organoids from 167 subjects with and 22 without CF, two parameters derived from a semi-automated image analysis protocol (rectal organoid morphology analysis, ROMA) fully discriminated CF subjects with two disease-causing mutations from non-CF subjects (p&lt;0.001). ROMA, feasible at all ages, can be centralised to improve standardisation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30209 - Paediatrics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Thorax

ISSN

0040-6376

e-ISSN

—

Volume of the periodical

76

Issue of the periodical within the volume

11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

1146-1149

UT code for WoS article

000709895600014

EID of the result in the Scopus database

2-s2.0-85104482171