Enhanced interrogation of degraded DNA from human skeletal remains: Increased genetic data recovery using the expanded CODIS loci, multiple sex determination markers, and consensus testing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064211%3A_____%2F19%3AW0000043" target="_blank" >RIV/00064211:_____/19:W0000043 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/19:10398894
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pubmed/31322643/" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/31322643/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1127/anthranz/2019/0931" target="_blank" >10.1127/anthranz/2019/0931</a>
Alternative languages
Result language
angličtina
Original language name
Enhanced interrogation of degraded DNA from human skeletal remains: Increased genetic data recovery using the expanded CODIS loci, multiple sex determination markers, and consensus testing
Original language description
Skeletal remains are among the most difficult types of samples encountered in forensic DNA casework and historical investigations due to prolonged exposure to environmental insults. DNA extracted from bone often is degraded, in low quantities, and contains co-purified inhibitors from the surrounding soil and/or burial vault material. When sexually dimorphic skeletal elements are not recovered, determining the sex of a decedent can be challenging. With unidentified human skeletal remains, genetic data often are evaluated in concert with anthropological analyses, as well as other types of metadata, to improve confidence in making associations or for positive identifications. This study evaluated a multi-faceted molecular genetic approach to increasing the amount of data that can be recovered from degraded skeletal remains. Results demonstrate that using a newer-generation multiplex (GlobalFiler (TM)) with an expanded set of highly discriminatory DNA markers - combined with co-amplification of three different sex-determining loci, one additional PCR cycle, and testing multiple cuttings from the same bone or multiple regions within a skeleton - can improve reliability and accuracy in skeletal remains identifications by providing data concordance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30501 - Forensic science
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ANTHROPOLOGISCHER ANZEIGER
ISSN
0003-5548
e-ISSN
—
Volume of the periodical
76
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
19
Pages from-to
333-351
UT code for WoS article
000489610600004
EID of the result in the Scopus database
2-s2.0-85072993262