Cytidine and dCMP Deaminases—Current Methods of Activity Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F25%3A10159401" target="_blank" >RIV/00098892:_____/25:10159401 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/26/16/8045" target="_blank" >https://www.mdpi.com/1422-0067/26/16/8045</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms26168045" target="_blank" >10.3390/ijms26168045</a>
Alternative languages
Result language
angličtina
Original language name
Cytidine and dCMP Deaminases—Current Methods of Activity Analysis
Original language description
Cytidine deaminase (CDA) and deoxycytidine monophosphate deaminase (DCTD) play crucial roles in pyrimidine metabolism, affecting DNA synthesis, cell cycle progression, and the efficacy of numerous nucleoside analog-based chemotherapeutics. Given their significance, accurate and sensitive measurement of their enzymatic activity is paramount for both fundamental biochemical research and clinical applications. This review provides a comprehensive overview of the methodologies used to assess CDA and DCTD activity, both established and emerging. We systematically categorize and discuss various approaches, including spectrophotometric, fluorimetric, liquid chromatography-based (Ultraviolet-Visible, fluorescence, and mass spectrometry), radiometric, and cell-based assays. For each method, we present its underlying principles, advantages, and limitations. Furthermore, we draw comparisons across the techniques to highlight their suitability for specific research questions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/EH22_008%2F0004644" target="_blank" >EH22_008/0004644: Saving lives through research in early cancer detection and prevention: Molecular, genomic and societal factors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1661-6596
e-ISSN
1422-0067
Volume of the periodical
26
Issue of the periodical within the volume
16
Country of publishing house
CH - SWITZERLAND
Number of pages
29
Pages from-to
8045
UT code for WoS article
001557742300001
EID of the result in the Scopus database
2-s2.0-105014287209