Monitoring the influence of antibiotic exposure using Raman spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061510" target="_blank" >RIV/00159816:_____/14:00061510 - isvavai.cz</a>
Alternative codes found
RIV/68081731:_____/14:00434960 RIV/00216224:14110/14:00073760
Result on the web
<a href="http://dx.doi.org/10.1117/12.2038595" target="_blank" >http://dx.doi.org/10.1117/12.2038595</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2038595" target="_blank" >10.1117/12.2038595</a>
Alternative languages
Result language
angličtina
Original language name
Monitoring the influence of antibiotic exposure using Raman spectroscopy
Original language description
Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with information of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
FS - Medical facilities, apparatus and equipment
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceedings of SPIE
ISBN
978-0-8194-9852-6
ISSN
0277-786X
e-ISSN
—
Number of pages
6
Pages from-to
"89390P"
Publisher name
Bellingham
Place of publication
United States
Event location
San Francisco
Event date
Mar 4, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000334098200015