Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F15%3A00062592" target="_blank" >RIV/00159816:_____/15:00062592 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/15:00080935

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkv198" target="_blank" >http://dx.doi.org/10.1093/nar/gkv198</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkv198" target="_blank" >10.1093/nar/gkv198</a>

Result language

angličtina

Original language name

Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates

Original language description

A variety of DNA lesions, secondary DNA structures or topological stress within the DNA template may lead to stalling of the replication fork. Recovery of such forks is essential for the maintenance of genomic stability. The structure-specific endonuclease Mus81-Mms4 has been implicated in processing DNA intermediates that arise from collapsed forks and homologous recombination. According to previous genetic studies, the Srs2 helicase may play a role in the repair of double-strand breaks and ssDNA gaps together with Mus81-Mms4. In this study, we show that the Srs2 and Mus81-Mms4 proteins physically interact in vitro and in vivo and we map the interaction domains within the Srs2 and Mus81 proteins. Further, we show that Srs2 plays a dual role in the stimulation of the Mus81-Mms4 nuclease activity on a variety of DNA substrates. First, Srs2 directly stimulates Mus81-Mms4 nuclease activity independent of its helicase activity. Second, Srs2 removes Rad51 from DNA to allow access of Mus81-Mms4 to cleave DNA. Concomitantly, Mus81-Mms4 inhibits the helicase activity of Srs2. Taken together, our data point to a coordinated role of Mus81-Mms4 and Srs2 in processing of recombination as well as replication intermediates.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

3626-73642

UT code for WoS article

000354722500025

EID of the result in the Scopus database

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