Toxicity of carbon dots - Effect of surface functionalization on the cell viability, reactive oxygen species generation and cell cycle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00064019" target="_blank" >RIV/00159816:_____/16:00064019 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/16:33159751 RIV/61989592:15110/16:33159751

Result on the web

<a href="http://dx.doi.org/10.1016/j.carbon.2015.12.027" target="_blank" >http://dx.doi.org/10.1016/j.carbon.2015.12.027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbon.2015.12.027" target="_blank" >10.1016/j.carbon.2015.12.027</a>

Result language

angličtina

Original language name

Toxicity of carbon dots - Effect of surface functionalization on the cell viability, reactive oxygen species generation and cell cycle

Original language description

Carbon dots (CDs) are fluorescent nanoprobes offering a great potential in biological and medical applications due to their superior biocompatibility compared to metal chalcogenide quantum dots (e.g., CdSe). Key factors determining their cytotoxicity and cellular/intracellular tracking involve chemical nature and charge of surface functional groups. For the first time, we present a comprehensive cytotoxic study including cell cycle analysis of carbon dots differing in surface functionalization, namely pristine CDs (CDs-Pri) with negative charge due to carboxylic groups, polyethyleneglycol modified dots with neutral charge (CDs-PEG), and polyethylenimine coated dots with a positive charge (CDs-PEI). The CDs in vitro toxicity was studied on standard mouse fibroblasts (NIH/3T3). The results suggest that neutral CDs-PEG are the most promising for biological applications as they do not induce any abnormalities in cell morphology, intracellular trafficking, and cell cycle up to concentrations of 300 μg mLMINUS SIGN 1. Negatively charged CDs-Pri arrested the G2/M phase of the cell cycle, stimulated proliferation and led to higher oxidative stress, however they did not enter the cell nucleus. In contrast, positively charged CDs-PEI are the most cytotoxic, entering into the cell nucleus and inducing the largest changes in G0/G1 phase of cell cycle, even at concentrations of around 100 μg mLMINUS SIGN 1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Carbon

ISSN

0008-6223

e-ISSN

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Volume of the periodical

99

Issue of the periodical within the volume

APR

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

238-248

UT code for WoS article

000369069800028

EID of the result in the Scopus database

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