Systemic inflammatory response syndrome is reduced by preoperative plasma-thrombo-leukocyte aphaeresis in a pig model of cardiopulmonary bypass

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065047" target="_blank" >RIV/00159816:_____/16:00065047 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/16:00092402 RIV/00209775:_____/16:N0000028

Result on the web

<a href="http://dx.doi.org/10.5507/bp.2016.010" target="_blank" >http://dx.doi.org/10.5507/bp.2016.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2016.010" target="_blank" >10.5507/bp.2016.010</a>

Result language

angličtina

Original language name

Systemic inflammatory response syndrome is reduced by preoperative plasma-thrombo-leukocyte aphaeresis in a pig model of cardiopulmonary bypass

Original language description

Objectives. The systemic inflammatory response syndrome (SIRS) after cardiac surgery with cardiopulmonary bypass (CPB) exacerbates organ dysfunction and increases postoperative mortality. The aim of this study was to reduce SIRS after CPB in a pig model by profoundly decreasing all blood defence factors (complement, coagulation and fibrinolytic and contact systems, leukocytes and thrombocytes) using pre-operative aphaeresis. Methods. Thirty -three pigs underwent 3 h of hypothermic CPB with 2 h of cardioplegic arrest, followed by 4 days of observation. One half of the sample underwent prebypass plasma-thrombo -leukocyte aphaeresis with the adjuvant leukofiltration. Results. In the control group, there were classical signs of SIRS (tachycardia, tachypnea and leukocytosis) postoperative - ly. There was also myocardial ischaemia and the need for inotropic support in 90% of the control animals. Neutrophils showed an increase in superoxide anion production ( P < 0.001), and surface neutral protease activity ( P < 0.001) and blood endotoxin levels increased ( P < 0.01) compared with preoperative levels. In contrast, in the aphaeretic group, there were no classical signs of SIRS; no myocardial ischaemia; minimum neutrophil production of the superoxide anion and protease activity were recorded ( P < 0.001); and endotoxin levels were also decreased ( P < 0.05) compared with the controls. In the control group, the haemodynamic problems associated with disconnecting from CPB correlated with the histologic findings in the myocardium (leukocyte endothelial adhesion and leukodiapedesis). Conclusions. Pre-operative plasma-thrombo -leukocyte aphaeresis significantly reduces the major symptoms of SIRS and organ dysfunction after 3 h of CPB without adverse effects, such as bleeding and infection, during the postopera - tive course.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FA - Cardiovascular diseases including cardio-surgery

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

160

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

399-406

UT code for WoS article

000392808100010

EID of the result in the Scopus database

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