Co-Expression of Cancer Stem Cell Markers Corresponds to a Pro-Tumorigenic Expression Profile in Pancreatic Adenocarcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065518" target="_blank" >RIV/00159816:_____/16:00065518 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/16:00095874 RIV/00209805:_____/16:00078579 RIV/65269705:_____/16:00065518

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0159255" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0159255</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0159255" target="_blank" >10.1371/journal.pone.0159255</a>

Result language

angličtina

Original language name

Co-Expression of Cancer Stem Cell Markers Corresponds to a Pro-Tumorigenic Expression Profile in Pancreatic Adenocarcinoma

Original language description

Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Its dismal prognosis is often attributed to the presence of cancer stem cells (CSCs) that have been identified in PDAC using various markers. However, the co-expression of all of these markers has not yet been evaluated. Furthermore, studies that compare the expression levels of CSC markers in PDAC tumor samples and in cell lines derived directly from those tumors are lacking. Here, we analyzed the expression of putative CSC markers-CD24, CD44, epithelial cell adhesion molecule (EpCAM), CD133, and nestin-by immunofluorescence, flow cytometry and quantitative PCR in 3 PDAC-derived cell lines and by immunohistochemistry in 3 corresponding tumor samples. We showed high expression of the examined CSC markers among all of the cell lines and tumor samples, with the exception of CD24 and CD44, which were enriched under in vitro conditions compared with tumor tissues. The proportions of cells positive for the remaining markers were comparable to those detected in the corresponding tumors. Co-expression analysis using flow cytometry revealed that CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells represented a significant population of the cells (range, 43 to 72%) among the cell lines. The highest proportion of CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells was detected in the cell line derived from the tumor of a patient with the shortest survival. Using gene expression profiling, we further identified the specific protumorigenic expression profile of this cell line compared with the profiles of the other two cell lines. Together, CD24(+)/CD44(+)/EpCAM(+)/CD133(+) cells are present in PDAC cell lines derived from primary tumors, and their increased proportion corresponds with a pro-tumorigenic gene expression profile.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

18

Pages from-to

"e0159255"

UT code for WoS article

000379579500112

EID of the result in the Scopus database

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