Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear beta-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00066929" target="_blank" >RIV/00159816:_____/17:00066929 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/17:00095409
Result on the web
<a href="http://dx.doi.org/10.1002/anie.201611193" target="_blank" >http://dx.doi.org/10.1002/anie.201611193</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201611193" target="_blank" >10.1002/anie.201611193</a>
Alternative languages
Result language
angličtina
Original language name
Different Structural Origins of the Enantioselectivity of Haloalkane Dehalogenases toward Linear beta-Haloalkanes: Open-Solvated versus Occluded-Desolvated Active Sites
Original language description
The enzymatic enantiodiscrimination of linear beta-haloalkanes is difficult because the simple structures of the substrates prevent directional interactions. Herein we describe two distinct molecular mechanisms for the enantiodiscrimination of the beta-haloalkane 2-bromopentane by haloalkane dehalogenases. Highly enantioselective DbjA has an open, solvent-accessible active site, whereas the engineered enzyme DhaA31 has an occluded and less solvated cavity but shows similar enantioselectivity. The enantioselectivity of DhaA31 arises from steric hindrance imposed by two specific substitutions rather than hydration as in DbjA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Angewandte Chemie-International Edition
ISSN
1433-7851
e-ISSN
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Volume of the periodical
56
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
4719-4723
UT code for WoS article
000398576000004
EID of the result in the Scopus database
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