Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067037" target="_blank" >RIV/00159816:_____/17:00067037 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/17:00098086 RIV/68081707:_____/17:00506842

Result on the web

<a href="http://dx.doi.org/10.18632/oncotarget.19016" target="_blank" >http://dx.doi.org/10.18632/oncotarget.19016</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.18632/oncotarget.19016" target="_blank" >10.18632/oncotarget.19016</a>

Result language

angličtina

Original language name

Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner

Original language description

The potentiation of the naturally limited regenerative capacity of the heart is dependent on an understanding of the mechanisms that are activated in response to pathological conditions such as hypoxia. Under these conditions, the expression of genes suggested to support cardiomyocyte survival and heart adaptation is triggered. Particularly important are changes in the expression of myosin heavy chain (MHC) isoforms. We propose here that alterations in the expression profiles of MHC genes are induced in response to hypoxia and are primarily mediated by hypoxia inducible factor (HIF). In in vitro models of mouse embryonic stem cell-derived cardiomyocytes, we showed that hypoxia (1% O2) or the pharmacological stabilization of HIFs significantly increased MHCbeta (Myh7) gene expression. The key role of HIF-1alpha is supported by the absence of these effects in HIF-1alpha-deficient cells, even in the presence of HIF-2alpha. Interestingly, ChIP analysis did not confirm the direct interaction of HIF-1alpha with putative HIF response elements predicted in the MHCalpha and beta encoding DNA region. Further analyses showed the significant effect of the mTOR signaling inhibitor rapamycin in inducing Myh7 expression and a hypoxia-triggered reduction in the levels of antisense RNA transcripts associated with the Myh7 gene locus. Overall, the recognized and important role of HIF in the regulation of heart regenerative processes could be highly significant for the development of novel therapeutic interventions in heart failure.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncotarget

ISSN

1949-2553

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

48

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

83684-83697

UT code for WoS article

000413030900026

EID of the result in the Scopus database

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