Standardized hiPSCs culture – vitronectin coating for cGMP compliant protocol

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067632" target="_blank" >RIV/00159816:_____/17:00067632 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Standardized hiPSCs culture – vitronectin coating for cGMP compliant protocol

Original language description

Background. Human induced pluripotent stem cells (hiPSCs) hold great promise for disease modeling, drug screening, toxicology testing, regenerative medicine and more. An important task for stem cell research translation into the clinic is culturing cells in completely defined conditions. Currently, many researchers culture hiPSCs on a surface coated with Matrigel(R), poorly defined protein mixture of animal origin. Alternatively, vitronectin can be used. Methods. We cultured two hiPSCs lines in mTeSRTM1 cell culture medium on a vitronectin-coated surface and compared their pluripotent markers expression and differentiation capacity in vitro to cells cultured on Matrigel(R) coated surface. Results. hiPSCs cultured on both surfaces expressed pluripotent markers and differentiated into all three germ layers. Conclusion. In this study, we tested culture method in completely defined conditions, thus compatible with current good manufacturing practice (cGMP). We demonstrate that vitronectin is a suitable Matrigel(R) replacement

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1213-8118

e-ISSN

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Volume of the periodical

161

Issue of the periodical within the volume

S1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

"S25"-"S28"

UT code for WoS article

000428945000005

EID of the result in the Scopus database

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