Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00068313" target="_blank" >RIV/00159816:_____/17:00068313 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/17:00095700 RIV/00209775:_____/17:N0000015
Result on the web
<a href="http://dx.doi.org/10.1159/000484524" target="_blank" >http://dx.doi.org/10.1159/000484524</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1159/000484524" target="_blank" >10.1159/000484524</a>
Alternative languages
Result language
angličtina
Original language name
Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
Original language description
Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR-DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment. (C) 2017 S. Karger AG, Basel
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal Of Molecular Microbiology And Biotechnology
ISSN
1464-1801
e-ISSN
—
Volume of the periodical
27
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
6
Pages from-to
350-355
UT code for WoS article
000426010400003
EID of the result in the Scopus database
—