Interleukin-4 induces a CD44(high)/CD49b(high) PC3 subpopulation with tumor-initiating characteristics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00068674" target="_blank" >RIV/00159816:_____/18:00068674 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.26607" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.26607</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcb.26607" target="_blank" >10.1002/jcb.26607</a>
Alternative languages
Result language
angličtina
Original language name
Interleukin-4 induces a CD44(high)/CD49b(high) PC3 subpopulation with tumor-initiating characteristics
Original language description
Pro- and anti-inflammatory cytokines may influence proliferation, migration, invasion, and other cellular events of prostate cancer (PCa) cells. The hyaluronan receptor CD44, which is regulated by Interleukin (IL)-4, is a prostate basal cell marker. CD44(high)/CD49b(high) expressing cells have been demonstrated to have tumor-initiating characteristics. Here, we aimed to analyze the effects of long-term IL-4 treatment on CD44/CD49b expression, migration, proliferation, and clonogenic potential of basal-like PCa cells. To this end PC3 cells were treated over 30 passages with 5ng/mL IL-4 (PC3-IL4) resulting in an increased population of CD44(high) expressing cells. This was concurrent with a clonal outgrowth of cuboid-shaped cells, with increased size and light absorbance properties. Flow cytometry revealed that the PC3-IL4 CD44(high) expressing subpopulation corresponds to the CD49b(high) population. Isolation of the PC3-IL4 CD44(high)/CD49b(high) subpopulation via fluorescence-associated cell sorting showed increased migrative, proliferative, and clonogenic potential compared to the CD44(low)/CD49b(low) subpopulation. In conclusion, IL-4 increases a PC3 subpopulation with tumor-initiating characteristics. Thus, IL-4, similar to other cytokines may be a regulator of tumor-initiation and hence, may present a suitable therapy target in combination with current treatment options.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF CELLULAR BIOCHEMISTRY
ISSN
0730-2312
e-ISSN
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Volume of the periodical
119
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
4103-4112
UT code for WoS article
000428417900030
EID of the result in the Scopus database
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