MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00068740" target="_blank" >RIV/00159816:_____/18:00068740 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/18:00102916

Result on the web

<a href="http://dx.doi.org/10.1007/s11255-018-1841-x" target="_blank" >http://dx.doi.org/10.1007/s11255-018-1841-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11255-018-1841-x" target="_blank" >10.1007/s11255-018-1841-x</a>

Result language

angličtina

Original language name

MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

Original language description

Currently, there is no accurate diagnostic molecular biomarker for renal cell carcinoma (RCC). The aim of this study was to assess the expression of microRNA-15a (miR-15a) in urine of patients with RCC and to evaluate its potential as a diagnostic molecular biomarker. In total, 67 patients with solid renal tumors were enrolled: clear-cell RCC (ccRCC, n = 22), papillary RCC (pRCC, n = 16), chromophobe RCC (chRCC, n = 14), oncocytoma (n = 8), papillary adenoma (n = 2) and angiomyolipoma (n = 5). MiRNA-15a expression levels measurement in urine were performed using qPCR. Urine of 15 healthy volunteers without kidney pathology was used as control. We observed a difference in mean miR-15a expression values in groups of pre-operative patients with RCC, benign renal tumors and healthy persons (2.50E-01 +/- 2.72E-01 vs 1.32E-03 +/- 3.90E-03 vs 3.36E-07 +/- 1.04E-07 RFU, respectively, p &lt; 0.01). There was no difference in miR-15a expression between ccRCC, pRCC and chRCC (p &gt; 0.05). Direct association between RCC size and miR-15a expression values was obtained (Pearson correlation coefficient-0.873). On the 8th day after nephrectomy, mean expression value in patients with RCC decreased by 99.53% (p &lt; 0.01). MiR-15a expression differentiated RCC from benign renal tumors with 98.1% specificity, 100% sensitivity at a cut-off value of 5.00E-06 RFU, with AUC-0.955. MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30217 - Urology and nephrology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International urology and nephrology

ISSN

0301-1623

e-ISSN

—

Volume of the periodical

50

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

851-859

UT code for WoS article

000431429000008

EID of the result in the Scopus database

—