A Simple Vacuum-Based Microfluidic Technique to Establish High-Throughput Organs-On-Chip and 3D Cell Cultures at the Microscale

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00071017" target="_blank" >RIV/00159816:_____/19:00071017 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/admt.201800319" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/admt.201800319</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/admt.201800319" target="_blank" >10.1002/admt.201800319</a>

Result language

angličtina

Original language name

A Simple Vacuum-Based Microfluidic Technique to Establish High-Throughput Organs-On-Chip and 3D Cell Cultures at the Microscale

Original language description

Microfluidic-based 3D cell culture and organs-on-chip have proved able to generate accurate in vitro models of human physiology. Their widespread application and adoption are however hampered by limited scalability and throughput. Here, a novel strategy is described to significantly enhance the throughput of microfluidic systems for 3D cell culture and organs-on-chips. A series of 3D culture chambers (up to 96 replicates) can be seeded with a single pipetting operation and a system of normally closed microfluidic valves ensures the resulting 3D microtissues are independent. Devices fabricated with this design principle are employed to perform 3D cultures of rat cardiac fibroblasts and profile two known drugs (doxorubicin, sotalol) in terms of cytotoxicity. In addition, human contractile cardiac microtissues is generated using iPSC-derived cardiac myocytes and functional assays on microtissues calcium transients after treatment with a known chronotropic drug (verapamil) are performed. The systems here described thus open up new perspective in the scalability of organs-on-chip and pave the way to multireplicate 3D cell cultures in microfluidics.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

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Continuities

R - Projekt Ramcoveho programu EK

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advanced Materials Technologies

ISSN

2365-709X

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

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UT code for WoS article

000455117500038

EID of the result in the Scopus database

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