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HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F20%3A00072959" target="_blank" >RIV/00159816:_____/20:00072959 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/20:00115466

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jchromb.2019.121942" target="_blank" >10.1016/j.jchromb.2019.121942</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster

  • Original language description

    A simple, sensitive HPLC-MS/MS method was developed and validated for the determination of lidocaine in skin and plasma of rats. The methods were established and validated assessing lower limit of quantitation (LLOQ), linearity, intra and inter-day precision and accuracy, selectivity, recovery and matrix effect. Chromatography was done on a Gemini column embedded with C18 stationary phase (50 mm x 2.0 mm, 5 mu m particle size), using a gradient with mobile phases consisting of 0.1% HCOOH in bidistilled water and 0.1% HCOOH in acetonitrile. The mass spectrometer worked with electrospray ionization in positive ion mode and selected reaction monitoring, using target ions m/z 235.10 for lidocaine and m/z 245.10 for lidocaine-d10, used as internal standard. Results: The linearity of the method was in the ranges of lidocaine concentrations 10.0-200.0 ng/mL for skin homogenate (accuracy 94.1-105.5%; R-2 &gt;= 0.998) and 0.025-2 ng/mL for plasma (accuracy 96.2-104.8%; R-2 &gt;= 0.996). The intra- and inter-day precision and accuracy determined on three quality control samples (20, 75 and 170 ng/mL for skin and 0.075, 0.4 and 1.5 ng/mL for plasma) were &lt;= 4.2% and 103.8-108.2% for skin and &lt;= 12.4% and 95.5-101.4% for plasma. The LLOQ was 10 ng/mL in skin homogenate and 0.025 ng/mL in plasma. The applicability of the method was demonstrated by measuring lidocaine in skin and plasma after exposure to medicated patches containing 5% lidocaine.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10609 - Biochemical research methods

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Chromatography B : Analytical technologies in the biomedical and life sciences

  • ISSN

    1570-0232

  • e-ISSN

  • Volume of the periodical

    1138

  • Issue of the periodical within the volume

    February

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

  • UT code for WoS article

    000510111600002

  • EID of the result in the Scopus database