HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F20%3A00072959" target="_blank" >RIV/00159816:_____/20:00072959 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/20:00115466
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S1570023219305598?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2019.121942" target="_blank" >10.1016/j.jchromb.2019.121942</a>
Alternative languages
Result language
angličtina
Original language name
HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster
Original language description
A simple, sensitive HPLC-MS/MS method was developed and validated for the determination of lidocaine in skin and plasma of rats. The methods were established and validated assessing lower limit of quantitation (LLOQ), linearity, intra and inter-day precision and accuracy, selectivity, recovery and matrix effect. Chromatography was done on a Gemini column embedded with C18 stationary phase (50 mm x 2.0 mm, 5 mu m particle size), using a gradient with mobile phases consisting of 0.1% HCOOH in bidistilled water and 0.1% HCOOH in acetonitrile. The mass spectrometer worked with electrospray ionization in positive ion mode and selected reaction monitoring, using target ions m/z 235.10 for lidocaine and m/z 245.10 for lidocaine-d10, used as internal standard. Results: The linearity of the method was in the ranges of lidocaine concentrations 10.0-200.0 ng/mL for skin homogenate (accuracy 94.1-105.5%; R-2 >= 0.998) and 0.025-2 ng/mL for plasma (accuracy 96.2-104.8%; R-2 >= 0.996). The intra- and inter-day precision and accuracy determined on three quality control samples (20, 75 and 170 ng/mL for skin and 0.075, 0.4 and 1.5 ng/mL for plasma) were <= 4.2% and 103.8-108.2% for skin and <= 12.4% and 95.5-101.4% for plasma. The LLOQ was 10 ng/mL in skin homogenate and 0.025 ng/mL in plasma. The applicability of the method was demonstrated by measuring lidocaine in skin and plasma after exposure to medicated patches containing 5% lidocaine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography B : Analytical technologies in the biomedical and life sciences
ISSN
1570-0232
e-ISSN
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Volume of the periodical
1138
Issue of the periodical within the volume
February
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
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UT code for WoS article
000510111600002
EID of the result in the Scopus database
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