Epilepsy miRNA Profile Depends on the Age of Onset in Humans and Rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F20%3A00073208" target="_blank" >RIV/00159816:_____/20:00073208 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/20:00533411 RIV/00216224:14740/20:00114449

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fnins.2020.00924/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fnins.2020.00924/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fnins.2020.00924" target="_blank" >10.3389/fnins.2020.00924</a>

Result language

angličtina

Original language name

Epilepsy miRNA Profile Depends on the Age of Onset in Humans and Rats

Original language description

Temporal lobe epilepsy (TLE) is a severe neurological disorder accompanied by recurrent spontaneous seizures. Although the knowledge of TLE onset is still incomplete, TLE pathogenesis most likely involves the aberrant expression of microRNAs (miRNAs). miRNAs play an essential role in organism homeostasis and are widely studied in TLE as potential therapeutics and biomarkers. However, many discrepancies in discovered miRNAs occur among TLE studies due to model-specific miRNA expression, different onset ages of epilepsy among patients, or technology-related bias. We employed a massive parallel sequencing approach to analyze brain tissues from 16 adult mesial TLE (mTLE)/hippocampal sclerosis (HS) patients, 8 controls and 20 rats with TLE-like syndrome, and 20 controls using the same workflow and categorized these subjects based on the age of epilepsy onset. All categories were compared to discover overlapping miRNAs with an aberrant expression, which could be involved in TLE. Our cross-comparative analyses showed distinct miRNA profiles across the age of epilepsy onset and found that the miRNA profile in rats with adult-onset TLE shows the closest resemblance to the profile in mTLE/HS patients. Additionally, this analysis revealed overlapping miRNAs between patients and the rat model, which should participate in epileptogenesis and ictogenesis. Among the overlapping miRNAs stand out miR-142-5p and miR-142-3p, which regulate immunomodulatory agents with pro-convulsive effects and suppress neuronal growth. Our cross-comparison study enhanced the insight into the effect of the age of epilepsy onset on miRNA expression and deepened the knowledge of epileptogenesis. We employed the same methodological workflow in both patients and the rat model, thus improving the reliability and accuracy of our results.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in neuroscience

ISSN

1662-453X

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

SEP 15

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

924

UT code for WoS article

000576142900001

EID of the result in the Scopus database

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