The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00077761" target="_blank" >RIV/00159816:_____/22:00077761 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/22:00128839
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2589004222017114?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2589004222017114?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2022.105439" target="_blank" >10.1016/j.isci.2022.105439</a>
Alternative languages
Result language
angličtina
Original language name
The role of bivalent ions in the regulation of D-loop extension mediated by DMC1 during meiotic recombination
Original language description
During meiosis, programmed DNA double-strand breaks (DSBs) are repaired by homologous recombination. DMC1, a conserved recombinase, plays a central role in this process. DMC1 promotes DNA strand exchange between homologous chromosomes, thus creating the physical linkage between them. Its function is regulated not only by several accessory proteins but also by bivalent ions. Here, we show that whereas calcium ions in the presence of ATP cause a conformational change within DMC1, stimulating its DNA binding and D-loop formation, they inhibit the extension of the invading strand within the D-loop. Based on structural studies, we have generated mutants of two highly conserved amino acids - E162 and D317 - in human DMC1, which are deficient in calcium regulation. In vivo studies of their yeast homologues further showed that they exhibit severe defects in meiosis, thus emphasizing the importance of calcium ions in the regulation of DMC1 function and meiotic recombination.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ISCIENCE
ISSN
2589-0042
e-ISSN
2589-0042
Volume of the periodical
25
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
27
Pages from-to
nestrankovano
UT code for WoS article
000923030100007
EID of the result in the Scopus database
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