A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00177016%3A_____%2F25%3AN0000136" target="_blank" >RIV/00177016:_____/25:N0000136 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/25:00140473

Result on the web

<a href="https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70084" target="_blank" >https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70084</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Original language description

Atomic force microscopy (AFM) has recently received increasing interest in molecular biology. This technique allows quick and reliable detection of biomolecules. However, studying RNA-protein complexes using AFM poses significant challenges. Here, we describe a simple and reliable method to visualize positively charged proteins bound to RNA that does not require metallic cations. This method allowed us to effectively detect and visualize Staufen-RNA complexes by height or logarithmic stiffness. The study of the mechanical properties is particularly important in the case of protein-coated RNA complexes, where RNA cannot be detected by height channel. In any case, it is necessary to compare AFM data with the data derived from other techniques like nuclear magnetic resonance, X-ray crystallography, cryogenic electron microscopy, and small-angle X-ray scattering. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Preparation and visualization of RNA-protein complex

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/EF18_046%2F0015974" target="_blank" >EF18_046/0015974: Upgrading Czech Infrastructure for Integrative Structural Biology</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Current Protocols

ISSN

2691-1299

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

001391965600001

EID of the result in the Scopus database

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