Colorectal cancer specific cytochrome P450 2W1 (CYP2W1): intracellular localization, glycosylation, and catalytic activity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F10%3A10080527" target="_blank" >RIV/00179906:_____/10:10080527 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Colorectal cancer specific cytochrome P450 2W1 (CYP2W1): intracellular localization, glycosylation, and catalytic activity
Original language description
Cytochrome P450 2W1 (CYP2W1) is expressed at high levels in colorectal cancer cells. We have shown previously that a higher tumor expression is associated with less survival. In this study, we characterize post-translational modification, inverted endoplasmic reticulum (ER) topology, and catalytic activity of CYP2W1. Immunofluorescence microscopy and cell surface biotinylation experiments revealed approx. 8% of the CYP2W1 on the cell surface. Despite the reverse orientation of CYP2W1 in the ER membrane,apparently making interactions with NADPH-cytochrome P450 reductase impossible, CYP2W1 in HEK293 cells was active in the metabolism of indoline substrates and in activation of aflatoxin B1 into cytotoxic products. The study identifies for the first timea cytochrome P450 with a luminal ER orientation and still retaining catalytic activity. The results suggest a possibility of using CYP2W1 as a drug target in the treatment of colon cancer using antibodies and/or specific CYP2W1 activated
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)