CysTRAQ - A combination of iTRAQ and enrichment of cysteinyl peptides for uncovering and quantifying hidden proteomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F12%3A10124637" target="_blank" >RIV/00179906:_____/12:10124637 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/12:43874649 RIV/00216208:11150/12:10124637
Result on the web
<a href="http://dx.doi.org/10.1016/j.jprot.2011.09.027" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2011.09.027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2011.09.027" target="_blank" >10.1016/j.jprot.2011.09.027</a>

Result language
angličtina
Original language name
CysTRAQ - A combination of iTRAQ and enrichment of cysteinyl peptides for uncovering and quantifying hidden proteomes
Original language description
Shotgun proteomics is capable of characterizing differences in both protein quality and quantity, and has been applied in various biomedical applications. Unfortunately, the high complexity and dynamic range of proteins in studied samples, clinical in particular, often hinders the identification of relevant proteins. Indeed, information-rich, low abundance proteins often remain undetected, whereas repeatedly reported altered concentrations in high abundance proteins are often ambiguous and insignificant. Several techniques have therefore been developed to overcome this obstacle and provide a deeper insight into the proteome. Here we report a novel approach, which enables iTRAQ reagent quantitation of peptides fractionated based on presence of a cysteine residue (thus CysTRAQ). For the first time, we prove that iTRAQ quantitation is fully compatible with cysteinyl peptide enrichment and is not influenced by the fractionation process. Moreover, the employment of the method combined with
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/NS10382" target="_blank" >NS10382: Identification of biomarkers of intraamniotic inflammation and the systemic fetal response syndrome in amniotic fluid: proteomic approach</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)