Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10145598" target="_blank" >RIV/00179906:_____/13:10145598 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/13:10145598
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300242/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300242/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201300242" target="_blank" >10.1002/jssc.201300242</a>

Result language
angličtina
Original language name
Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography
Original language description
The reduction of analysis time, cost, and improvement of separation efficiency are the main requirements in the development of high-throughput assay methods in bioanalysis. It can be achieved either by ultra-high-performance liquid chromatography (UHPLC)using stationary phases with small particles (<2 micro m) at high back pressures or by using opposite direction - monolithic stationary phases with low back pressures. The application of new types of monolithic stationary phases for UHPLC is a novel idea combining these two different paths. The aim of this work was to test the recently introduced second-generation of monolithic column Chromolith (R) HighResolution for UHPLC analysis of liposoluble vitamins in comparison with core-shell and fully poroussub-2 micro m columns with different particle sizes, column lengths, and shapes. The separation efficiency, peak shape, resolution, time of analysis, consumption of mobile phase, and lifetime of columns were calculated and compared. The
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Establishment of Research Team Focused on Experimental and Applied Biopharmacy</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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