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Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10188841" target="_blank" >RIV/00179906:_____/13:10188841 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11150/13:10188841

  • Result on the web

    <a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >http://dx.doi.org/10.3109/00365548.2013.795656</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >10.3109/00365548.2013.795656</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis

  • Original language description

    Background: The aims of this work were to replace the obsolete PCR method for the laboratory diagnosis of the acute form of leptospirosis using the G1, G2 an B64 I, B64 II primers, and to improve the PCR detection time.Methods: We introduced a real-timePCR method for the detection of the gene encoding the surface lipoprotein LipL32 of pathogenic Leptospira into our laboratory diagnosis of the acute fom of leptospirosis. The positive and negative analytical specificities of the real-time PCR method wereboth equal to 100%; the detection limit was determined to be 1-5 genome copies/1 ml of liquid biological material.The method was further validated on 230 laboratory strains of leptospires. Results: All laboratory strains of pathogenic Leptospira were evaluated as LipL32-positive and all non-pathogenic strains as LipL32-negative. In addition, 455 biological materials (253 plasma, 121 urine, 72 cerebrospinal fluid (CSF), 7 bronchoalveolar lavage, and 2 sputum) from 295 patients with suspe

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scandinavian Journal of Infectious Diseases

  • ISSN

    0036-5548

  • e-ISSN

  • Volume of the periodical

    45

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    NO - NORWAY

  • Number of pages

    7

  • Pages from-to

    593-599

  • UT code for WoS article

    000321789300003

  • EID of the result in the Scopus database