Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10188841" target="_blank" >RIV/00179906:_____/13:10188841 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/13:10188841
Result on the web
<a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >http://dx.doi.org/10.3109/00365548.2013.795656</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/00365548.2013.795656" target="_blank" >10.3109/00365548.2013.795656</a>
Alternative languages
Result language
angličtina
Original language name
Real-time PCR method for the detection of the gene encoding surface lipoprotein LipL32 of pathogenic Leptospira: use in the laboratory diagnosis of the acute form of leptospirosis
Original language description
Background: The aims of this work were to replace the obsolete PCR method for the laboratory diagnosis of the acute form of leptospirosis using the G1, G2 an B64 I, B64 II primers, and to improve the PCR detection time.Methods: We introduced a real-timePCR method for the detection of the gene encoding the surface lipoprotein LipL32 of pathogenic Leptospira into our laboratory diagnosis of the acute fom of leptospirosis. The positive and negative analytical specificities of the real-time PCR method wereboth equal to 100%; the detection limit was determined to be 1-5 genome copies/1 ml of liquid biological material.The method was further validated on 230 laboratory strains of leptospires. Results: All laboratory strains of pathogenic Leptospira were evaluated as LipL32-positive and all non-pathogenic strains as LipL32-negative. In addition, 455 biological materials (253 plasma, 121 urine, 72 cerebrospinal fluid (CSF), 7 bronchoalveolar lavage, and 2 sputum) from 295 patients with suspe
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scandinavian Journal of Infectious Diseases
ISSN
0036-5548
e-ISSN
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Volume of the periodical
45
Issue of the periodical within the volume
8
Country of publishing house
NO - NORWAY
Number of pages
7
Pages from-to
593-599
UT code for WoS article
000321789300003
EID of the result in the Scopus database
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