New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10191186" target="_blank" >RIV/00179906:_____/13:10191186 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/13:10191186 RIV/00216208:11160/13:10191186
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300553/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300553/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201300553" target="_blank" >10.1002/jssc.201300553</a>

Result language
angličtina
Original language name
New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972
Original language description
Biomarkers, 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra-HPLC with MS/MS methodology for the analysis of 25-hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R-2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2, respectively. Intra-assay precision (%RSD) was lower than 4.5%, and inter-assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non-agenarian patients, patients with familial hypercholesterolemia and patients suffering from age-related ma
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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