Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F16%3A10330516" target="_blank" >RIV/00179906:_____/16:10330516 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/16:10330516

Result on the web

<a href="http://biomed.papers.upol.cz/pdfs/bio/2016/04/16.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2016/04/16.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2016.032" target="_blank" >10.5507/bp.2016.032</a>

Result language

angličtina

Original language name

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Original language description

Aims. Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest presence of genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been quantified sufficiently. Presented study aims to contribute to the quantification of the genotoxic risk. Methods. The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n=29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. Results. The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P=0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3.4-10.5) vs. 20.7 (19.3; 14.2-24.6) %tDNA. The level of total number of aberrated cells (P<0.001) and structurally aberrated cells (P<0.001) increased significantly. Conclusion. The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated the presence of genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for Czech healthy adults. Therefore, the level of genotoxic risk seems to be low. On the other hand, it is evident that Goeckerman therapy represents a further contribution to the lifetime load of genotoxic factors.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FM - Hygiene

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

—

Volume of the periodical

160

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

553-558

UT code for WoS article

000392810500016

EID of the result in the Scopus database

2-s2.0-85006274753