Proteomic analysis of early mid-trimester amniotic fluid does not predict spontaneous preterm delivery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F16%3A10331179" target="_blank" >RIV/00179906:_____/16:10331179 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/16:10331179 RIV/60162694:G44__/16:43875602

Result on the web

<a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155164" target="_blank" >http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155164</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0155164" target="_blank" >10.1371/journal.pone.0155164</a>

Result language

angličtina

Original language name

Proteomic analysis of early mid-trimester amniotic fluid does not predict spontaneous preterm delivery

Original language description

Objective The aim of this study was to identify early proteomic biomarkers of spontaneous preterm delivery (PTD) in mid-trimester amniotic fluid from asymptomatic women. Methods This is a case-cohort study. Amniotic fluid from mid-trimester genetic amniocentesis (14-19 weeks of gestation) was collected from 2008 to 2011. The analysis was conducted in 24 healthy women with subsequent spontaneous PTD (cases) and 40 randomly selected healthy women delivering at term (controls). An exploratory phase with proteomics analysis of pooled samples was followed by a verification phase with ELISA of individual case and control samples. Results The median (interquartile range (IQR: 25th; 75th percentiles) gestational age at delivery was 35+5 (33+6-36+6) weeks in women with spontaneous PTD and 40+0 (39+1-40+5) weeks in women who delivered at term. In the exploratory phase, the most pronounced differences were found in C-reactive protein (CRP) levels, that were approximately two-fold higher in the pooled case samples than in the pooled control samples. However, we could not verify these differences with ELISA. The median (25th; 75th IQR) CRP level was 95.2 ng/mL (64.3; 163.5) in women with spontaneous PTD and 86.0 ng/mL (51.2; 145.8) in women delivering at term (p = 0.37; t-test). Conclusions Proteomic analysis with mass spectrometry of mid-trimester amniotic fluid suggests CRP as a potential marker of spontaneous preterm delivery, but this prognostic potential was not verified with ELISA.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FK - Gynaecology and obstetrics

OECD FORD branch

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Project

<a href="/en/project/NT13599" target="_blank" >NT13599: Characterization of the diagnostic potential of native polypeptides in amniotic fluid</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

000376880200008

EID of the result in the Scopus database

2-s2.0-84971010983