Oxaliplatin and irinotecan induce heterogenous changes in the EMT markers of metastasizing colorectal carcinoma cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F18%3A10379327" target="_blank" >RIV/00179906:_____/18:10379327 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/18:10379327 RIV/00216208:11160/18:10379327
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0014482718303033" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0014482718303033</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.yexcr.2018.05.032" target="_blank" >10.1016/j.yexcr.2018.05.032</a>
Alternative languages
Result language
angličtina
Original language name
Oxaliplatin and irinotecan induce heterogenous changes in the EMT markers of metastasizing colorectal carcinoma cells
Original language description
In patients with advanced colorectal cancer (CRC), surgery is complemented with systemic therapy - chemotherapy and radiochemotherapy. Although the patients' overall survival has been significantly improved, tumor resistance is still a frequent cause of chemotherapy failure. Several factors contribute to chemoresistance of tumor cells including changes related with epithelial-mesenchymal transition (EMT). The present study was designed to verify the presence of EMT markers in paired CRC primary cell lines obtained from primary tumor sites and lymph node metastases of three patients and to investigate the effect of irinotecan and oxaliplatin treatment on these markers as well. The samples of the higher stage of CRC and positive for angioinvasion were selected and qPCR, western blot analysis, migration assay, cytotoxicity testing was performed. Results confirmed the increased expression of several markers characteristic of EMT and invasiveness in lymph node metastatic cells, with a significant variability between individual samples. Irinotecan and oxaliplatin decreased migration activity of the cells and to the varying degree affected the expression of EMT and invasiveness markers. In conclusion, in CRC EMT is present in metastatic cells over a phenotypic continuum whose expression is altered heterogeneously upon irinotecan and oxaliplatin treatment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GA17-10331S" target="_blank" >GA17-10331S: Elucidation of role of cadherins and EMT in the development of chemotherapy resistance in metastatic colorectal cancer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Cell Research
ISSN
0014-4827
e-ISSN
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Volume of the periodical
369
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
295-303
UT code for WoS article
000442063200013
EID of the result in the Scopus database
2-s2.0-85047521815