Enzymatic Isolation, Amplification and Characterization of Dental Pulp Stem Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F19%3A10398958" target="_blank" >RIV/00179906:_____/19:10398958 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/19:10398958
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5iJoHjodRI" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5iJoHjodRI</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Enzymatic Isolation, Amplification and Characterization of Dental Pulp Stem Cells
Original language description
The dental pulp represents an easily acces-sible source of adult dental pulp stem cells (DPSCs). The preferred approach to DPSC isolation is enzy-matic digestion. However, the duration of the enzymat-ic activity is crucial. The purpose of this study was to isolate the DPSC populations using this method, characterize their biological properties and prolifer-ation capacity, and to determine their ability to dif-ferentiate into mature cells. Before enzymatic diges-tion using 0.05% trypsin, we used the homogenization method in order to obtain a fine homogenate from the solid pulp tissue. The stem cells were cultivated in modified cultivation medium for mesenchymal adult progenitor cells containing 2% foetal bovine serum, growth factors and insulin-transferrin-sele-nium supplement. We were successfully able to iso-late 10 populations of DPSCs. The vitality of DPSCs did not drop below 90 %. However, the DPSCs showed a significant decrease in the relative telomere length number with increasing passaging (P < 0.05). Isolated DPSCs highly expressed the CD markers: CD29, CD44, CD90, CD13, CD73 and CD166. In con-trast, CD markers CD31, CD106, CD34 and CD45 were negative or low positive. We confirmed the high osteogenic and chondrogenic potential of the isolated stem cells. Isolated DPSCs did not show signs of cell degeneration or spontaneous differentiation during the entire cultivation. In addition, we were able to shorten the enzyme activity duration, and we were the first to demonstrate trypsin as the enzyme used for the enzymatic digestion method with the viability over 90 % of isolated DPSCs using this method.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia Biologica
ISSN
0015-5500
e-ISSN
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Volume of the periodical
65
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
124-133
UT code for WoS article
000500271800002
EID of the result in the Scopus database
2-s2.0-85073656780