Qualitative and quantitative changes in the use of cryo-technology during the 70-year history of the tissue bank, university hospital Hradec Kralove

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10477126" target="_blank" >RIV/00179906:_____/23:10477126 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Qualitative and quantitative changes in the use of cryo-technology during the 70-year history of the tissue bank, university hospital Hradec Kralove

Original language description

The authors review the changes in the use of cryotechnology during the 70-year history of the Tissue Bank. The Tissue Bank of the University Hospital Hradec Králové was established by prof. Rudolf Klen, M.D., DSc. in 1952. At the beginning of its activity hypothermic preservation and freezing without cryoprotection was used for storage of solid tissue grafts such as corneas, skin bone and cartilage. Introduction of cryo-technology was related to the start of the programme of cryopreservation of tissue culture cells at the end of 60&apos;s of the last century. Controlled rate freezing in the vapour phase of liquid nitrogen and storage in small biological liquid nitrogen containers (STS Ricany, Czechoslovakia) was used. The use of cryo-technology expanded after the start of the programme of the haematopoietic progenitor cell (HPC) transplantation in 1989. The Bank was equipped with the programmable freezer (Planer Biomed, UK) and with the biological liquid nitrogen container BK 200 A (Ferox, Decin). This vessel was used for storage of small metal containers of own construction (VUPCHT, Hradec Kralove, Czechoslovakia) with cryopreserved HPC in the volume of 120 mL In the years 1992-94 the metal containers were replaced by disposable plastic bags. The new cryo-storage facility opened in 2002 was equipped with large biological containers with automatic filling (MVE, USA), continuous recording of temperature and the parameters of the environment - oxygen level, temperature, relative humidity. Since that time the consumption of liquid nitrogen has been increasing as well as the number of stored cryopreserved HPC units.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30205 - Hematology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Cryogenics 2023. The 17th IIR International Conference, Dresden, Germany, April 25-28, 2023. Proceedings

ISBN

978-2-36215-053-1

ISSN

0151-1637

e-ISSN

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Number of pages

9

Pages from-to

166-174

Publisher name

International Institute of Refrigeration

Place of publication

Paris

Event location

Dresden

Event date

Apr 25, 2023

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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