Use of autologous platelet-rich plasma for skin graft preservation: an experimental comparative study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F24%3A10481167" target="_blank" >RIV/00179906:_____/24:10481167 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/25:00563641
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Iu9ukdUnJt" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Iu9ukdUnJt</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.62347/MLIW4300" target="_blank" >10.62347/MLIW4300</a>
Alternative languages
Result language
angličtina
Original language name
Use of autologous platelet-rich plasma for skin graft preservation: an experimental comparative study
Original language description
Objective: In this experimental study, we aimed to determine whether platelet-rich plasma (PRP) is a suitable preservative for dermo-epidermal grafts. An additional objective was to investigate how long grafts can be stored without biological degradation. Methods: We compared pig skin graft preservation using PRP versus saline solution and crystalloid Custodiol (R), which is used for hypothermic preservation of organs for transplantation. Grafts (10 x 10 mm) were placed on gauze impregnated with one of the tested solutions, and stored for 3, 7, 11, and 15 days at a constant temperature of 4 degrees C. We evaluated a total of 240 pig skin samples: 120 by histopathology and 120 by fluorescence optical microscopy. Results: Overall, Custodiol (R) solution appeared to be the best medium for preservation of dermo-epidermal grafts, with beneficial properties manifested on days 7 and 11. Although we expected PRP to be a better preservative than saline, this was not confirmed by our results, as we found no significant difference between these two media. In fact, by day 3, the histopathological results were better with standard saline solution than with PRP. On day 15, with each tested solution, some samples showed histological changes that are incompatible with graft viability. Conclusion: Overall, Custodiol (R) appears to be the best medium for dermo-epidermal graft preservation. Moreover, the present findings suggest a maximum graft storage time of 11 days in all of the tested solutions. We do not recommend using grafts stored for 15 days, due to isolated signs of graft biodegradation with all solutions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Burns and Trauma
ISSN
2160-2026
e-ISSN
2160-2026
Volume of the periodical
14
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
38-47
UT code for WoS article
001229314300001
EID of the result in the Scopus database
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