Antioxidant versus prooxidant properties of the flavonoid, galangin: ROS scavenging activity, flavonoid-DNA interaction, copper-catalyzed Fenton reaction and DNA damage study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F24%3A10481313" target="_blank" >RIV/00179906:_____/24:10481313 - isvavai.cz</a>

Alternative codes found

RIV/62690094:18470/24:50021425

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ifsbBZJB-t" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=ifsbBZJB-t</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jafr.2024.101112" target="_blank" >10.1016/j.jafr.2024.101112</a>

Result language

angličtina

Original language name

Antioxidant versus prooxidant properties of the flavonoid, galangin: ROS scavenging activity, flavonoid-DNA interaction, copper-catalyzed Fenton reaction and DNA damage study

Original language description

Flavonoids are natural antioxidants, found in vegetables, fruits, flowers, coffee, tea, wine, and other foodstuffs. They are well known for their health benefits, and various clinical trials have determined that a sufficient intake of flavonoids is related to lower mortality from cardiovascular diseases, metabolic diseases, and cancer. In addition, flavonoids are of great current interest in regard to sporting activities, in that the intake of these compounds by sportsmen may both shorten their recovery periods and improve their overall health status. In this work, we present results demonstrating the antioxidant, and also prooxidant, properties of the flavonoid, galangin, which is currently under intense investigation as a health supplement. The prooxidant environment has been simulated using Cu -catalyzedFenton and Cu-Ascorbate systems. UV-Vis spectroscopy revealed that galangin is coordinated to Cu(II) through oxygen donor atoms from both the benzoyl (4-CO/5-OH groups) and the cinnamoyl (3-OH/4-CO) moieties of the molecule; however, the interaction occurs preferentially via the cinnamoyl unit. The radical scavenging activity of galangin and Cu(II)-galangin complexes were studied using the ABTS assay, which confirmed an inhibitory/scavenging activity of the systems studied, but chelation resulted in only a modest increase in activity. Electronic absorption titrations showed that both free galangin and the Cu(II)-galangin complex interact with DNA through hydrogen -bonding and van der Waals interactions. The values evaluated for the binding constants (K) indicate an intermediate binding strength between the given flavonoid systems and DNA. The results from gel electrophoresis have shown that, in the ROS-producing prooxidant systems, galangin provided a concentration -dependent protective effect against DNA damage via both Cu(II)-chelation and ROS-scavenging mechanisms. The ROS scavengers, L-his- tidine, DMSO, and SOD, confirmed the formation of singlet oxygen (O-1(2)), hydroxyl radicals ((OH)-O-center dot), and superoxide radical anions (O-2(center dot-)).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30100 - Basic medicine

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Agriculture and Food Research

ISSN

2666-1543

e-ISSN

2666-1543

Volume of the periodical

16

Issue of the periodical within the volume

JUN

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

101112

UT code for WoS article

001214719000001

EID of the result in the Scopus database

2-s2.0-85188945286