PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F03%3A%230000255" target="_blank" >RIV/00209775:_____/03:#0000255 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2003.007.19x/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1469-0691.2003.007.19x/pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia

Original language description

To assess the usefulness of polymerase chain reaction PCR) assays in the diagnosis of fungal infections in immunocompromised patients. A rapid and sensitive PCR-based assay for the detection and identification of fungal pathogens was designed and applicability of this method was investigated in a group of children with cancer and febrile neutropenia (FN). The ITS2 sequences and adjacent regions of 40 fungal pathogens were analyzed and primers for detection of all analyzed fungal species were designed. Amplification product lenght polymorphism (APLP) and restriction fragment lenght polymorphism (RFLP) generated genus- or species-specific patterns. The sensitivity of the method was approximately three cells of Candida albicans per 1 mL of blood. The results were available within 8 h after sample collection. The method was tested on 53 blood samples and one lung biopsy sample form 24 children with cancer and febrile neutropenia (FN). The PCR assay detected fungal DNA in 25 clinical sample

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/NM6870" target="_blank" >NM6870: Detection of origin of infection after extensive surgical treatment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical microbiology and infection

ISSN

1469-0691

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

12

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

1191-1202

UT code for WoS article

000187255800004

EID of the result in the Scopus database

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