Rheumatoid arthritis and bronchial asthma are associated with chnages in PLAUR gene expression in monocytes and macrophages

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F25%3AN0000007" target="_blank" >RIV/00209775:_____/25:N0000007 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0378111925006262?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0378111925006262?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/jgene.2025.149837" target="_blank" >10.1016/jgene.2025.149837</a>

Result language

angličtina

Original language name

Rheumatoid arthritis and bronchial asthma are associated with chnages in PLAUR gene expression in monocytes and macrophages

Original language description

The plasminogen activation system plays an important role in the pathogenesis of both rheumatoid arthritis (RA) and bronchial asthma (BA). Elevated levels of system components, including the urokinase plasminogen activator receptor (uPAR), have been observed in these conditions. The PLAUR gene, encoding uPAR, undergoes alternative splicing due to the presence of cassette exons, producing two major isoforms: membrane-bound uPAR (muPAR) and soluble uPAR (suPAR), distinguished by their membrane association determined by terminal exon usage. Given the increased suPAR levels reported in the plasma of RA and BA patients, we hypothesized that altered PLAUR expression, beyond post-translational muPAR cleavage, could contribute to disease susceptibility and progression. To test this, we analyzed PLAUR transcript levels and alternative splicing patterns in monocytes and macrophages (M0 and M1 phenotypes) isolated from 37 healthy volunteers and patients with RA (29 in total) and BA (31 in total), stratified by disease severity.Quantitative RT-PCR analysis revealed significantly elevated PLAUR expression in monocytes and M0 macrophages from both RA and BA patients compared to healthy controls. In mild BA, this increase was limited to overall expression, while severe BA was additionally characterized by an enrichment of low-abundance splicing isoforms: suPAR lacking exon 6 and muPAR lacking exon 5.These findings suggest that both increased PLAUR expression and alternative splicing events may contribute to the immunopathology of RA and BA. The relative abundance of suPAR isoforms may serve as a potential biomarker for disease progression, though further functional validation is necessary to elucidate their clinical relevance.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30230 - Other clinical medicine subjects

Project

<a href="/en/project/NU21-05-00438" target="_blank" >NU21-05-00438: The role of uPAR alternative isoforms in immunopathological response</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Gene

ISSN

—

e-ISSN

1879-0038

Volume of the periodical

972

Issue of the periodical within the volume

149837

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

1-10

UT code for WoS article

001603887400001

EID of the result in the Scopus database

2-s2.0-105020586845