Characterization of specific p63 and p63-N-terminal isoform antibodies and their application for immunohistochemistry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F13%3A%230000423" target="_blank" >RIV/00209805:_____/13:#0000423 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007%2Fs00428-013-1459-4" target="_blank" >http://link.springer.com/article/10.1007%2Fs00428-013-1459-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00428-013-1459-4" target="_blank" >10.1007/s00428-013-1459-4</a>

Result language

angličtina

Original language name

Characterization of specific p63 and p63-N-terminal isoform antibodies and their application for immunohistochemistry

Original language description

The TP63 gene gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (?Np63) of N-terminal p53-like transactivation domain. Immunohistochemistry for p63 has clinical value for certain tumour types, but investigations have been hampered by a lack of well characterized antibodies and the inability to discriminate between these N-terminal isoforms with opposite functional properties. We have characterized a series of monoclonal antibodies to recombinant human TAp63 and two commercial p63 monoclonals by Western blot, immunostaining and phage display epitope mapping. 28 of 29 (96.6 %) novel monoclonals that recognized all p63 isoforms showed substantial cross-reactivity with p73, as did the commercial antibody, 4A4. One novel clone, PANp63-6.1, showed slight cross-reaction with p73 by Western blotting but not immunohistochemistry and the SFI-6 monoclonal did not cross-react with p73 or p53. Phage display revealed that PANp63-6.1 epitope

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virchows Archiv

ISSN

0945-6317

e-ISSN

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Volume of the periodical

463

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

415-425

UT code for WoS article

000323904700007

EID of the result in the Scopus database

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