Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F13%3A%230000472" target="_blank" >RIV/00209805:_____/13:#0000472 - isvavai.cz</a>
Result on the web
<a href="http://www.tandfonline.com/doi/abs/10.1080/01635581.2013.756530#.U2EN1KJaKec" target="_blank" >http://www.tandfonline.com/doi/abs/10.1080/01635581.2013.756530#.U2EN1KJaKec</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/01635581.2013.756530" target="_blank" >10.1080/01635581.2013.756530</a>

Result language
angličtina
Original language name
Identification of microRNAs regulated by isothiocyanates and association of polymorphisms imide their target sites with risk of sporadic colorectal cancer
Original language description
Sporadic colorectal cancer (CRC) is a typical multifactorial disease. Isothiocyanates (ITC) have been recently shown to inhibit development of CRC in many experimental models. MicroRNAs (miRNAs) are short noncoding RNAs that posttranscriptionally regulate gene expression through binding to 3 untranslated regions (3UTR) of target mRNAs. MiRNAs are regulated by natural agents, ITCs included. In our study, using global expression profiling based on TaqMan Low-Density Arrays, we identified 3 common miRNAs (miR-155, miR-23b, miR-27b) regulated by ITCs (sulforaphane, iberin) in colonic epithelial cell lines NCM460 and NCM356. In silico predictions allowed us to find 9 relevant single nucleotide polymorphisms (SNPs) localized within the 3UTRs of genes (AGTR1,TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs. Finally, we observed that homozygous CC genotype of DICER1, rs1057035, was significantly associated with decreased risk of CRC (odds
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Project
<a href="/en/project/NS10352" target="_blank" >NS10352: MicroRNAs significance in chemoprotective effects of phytochemicals contained in Brassica vegetables and association of their binding sites polymorphisms with risk of colorectal cancer</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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