Discovery of a novel ligand that modulates the protein?protein interactions of the AAA+ superfamily oncoprotein reptin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F15%3A%230000612" target="_blank" >RIV/00209805:_____/15:#0000612 - isvavai.cz</a>

Result on the web

<a href="http://pubs.rsc.org/en/content/articlepdf/2015/sc/c4sc03885a" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2015/sc/c4sc03885a</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4sc03885a" target="_blank" >10.1039/c4sc03885a</a>

Result language

angličtina

Original language name

Discovery of a novel ligand that modulates the protein?protein interactions of the AAA+ superfamily oncoprotein reptin

Original language description

Developing approaches to discover protein?protein interactions (PPIs) remains a fundamental challenge. A chemical biology platform is applied here to identify novel PPIs for the AAA+ superfamily oncoprotein reptin. An in silico screen coupled with chemical optimization provided Liddean, a nucleotide-mimetic which modulates reptin's oligomerization status, protein-binding activity and global conformation. Combinatorial peptide phage library screening of Liddean-bound reptin with next generation sequencing identified interaction motifs including a novel reptin docking site on the p53 tumor suppressor protein. Proximity ligation assays demonstrated that endogenous reptin forms a predominantly cytoplasmic complex with its paralog pontin in cancer cells andLiddean promotes a shift of this complex to the nucleus. An emerging view of PPIs in higher eukaryotes is that they occur through a striking diversity of linear peptide motifs. The discovery of a compound that alters reptin's protein int

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical science

ISSN

2041-6539

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

3109-3116

UT code for WoS article

000353223100055

EID of the result in the Scopus database

2-s2.0-84928152626