Hydrogen/deuterium exchange mass spectrometry and its utilization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F15%3A%230000659" target="_blank" >RIV/00209805:_____/15:#0000659 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jri.2015.09.004" target="_blank" >http://dx.doi.org/10.1016/j.jri.2015.09.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jri.2015.09.004" target="_blank" >10.1016/j.jri.2015.09.004</a>

Result language

angličtina

Original language name

Hydrogen/deuterium exchange mass spectrometry and its utilization

Original language description

Hydrogen/deuterium exchange connected with mass spectrometry is increasingly applied for the interrogation of protein conformation, mapping protein dynamics, identification of protein-ligand interaction sites, and allosteric conformation changes. The dynamics of protein changes is determined with the m/z value of deuterated and non-deuterated protein or percentage of deuterium incorporation for the digested peptides. Hydrogen/deuterium exchange data are processed with selected software and finally quaternary structure of protein is visualized showing how different protein and ligand chains hook up with each other. Obtained results can help understand how protein interacts with its ligand and elucidate the role of this complex in a living organism. Utilization of this method is demonstrated by the amyloid-beta peptide aggregation associated with Alzheimer´s disease; determination of the structure toxin-co-regulated pili Vibrio cholerae in connection with its pathogenesis or revelation o

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Military medical science letters

ISSN

0165-0378

e-ISSN

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Volume of the periodical

84

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

146-151

UT code for WoS article

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EID of the result in the Scopus database

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