ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F16%3AN0000046" target="_blank" >RIV/00209805:_____/16:N0000046 - isvavai.cz</a>

Result on the web

<a href="http://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2808-x" target="_blank" >http://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2808-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12885-016-2808-x" target="_blank" >10.1186/s12885-016-2808-x</a>

Result language

angličtina

Original language name

ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells

Original language description

p63, a member of the p53 protein family, plays key roles in epithelial development and carcinogenesis. In breast cancer, p63 expression has been found predominantly in basal-A (epithelial-type) triplenegative breast carcinomas (TNBC). To investigate the functional role of p63 in basal-A TNBC, we created MDA-MB-468 cell lines with inducible expression of the two major N-terminal p63 isoforms, TAp63α and ΔNp63α. TAp63α did not have significant effect on gene expression profile and cell phenotype, whilst the main effect of ΔNp63α was reduction of cell adhesion. Gene expression profiling revealed genes involved in cell adhesion and migration whose expression relies on overexpression of ΔNp63α. Reduced cell adhesion also led to decreased cell proliferation in vitro and in vivo. Similar data were obtained in another basal-A cell line, BT-20, but not in BT-549 basal-B (mesenchymal-like) TNBC cells. In basal-A TNBC cells, ΔNp63α has much stronger effects on gene expression than TAp63α. Although p63 is mentioned mostly in connection with breast cell differentiation and stem cell regulation, we showed that a major effect of p63 is regulation of cell adhesion, a process important in metastasis and invasion of tumour cells. That this effect is not seen in mesenchymal-type TNBC cells suggests lineage-dependent functions, mirroring the expression of ΔNp63α in primary human breast cancers.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Cancer

ISSN

1471-2407

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

10 October 2016

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

782

UT code for WoS article

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EID of the result in the Scopus database

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