Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F18%3A00078023" target="_blank" >RIV/00209805:_____/18:00078023 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/18:00105867 RIV/65269705:_____/18:00069425
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0003267018309723" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267018309723</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2018.08.020" target="_blank" >10.1016/j.aca.2018.08.020</a>

Result language
angličtina
Original language name
Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples
Original language description
Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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