A single synonymous mutation determines the phosphorylation and stability of the nascent protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F19%3A00078030" target="_blank" >RIV/00209805:_____/19:00078030 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/jmcb/advance-article/doi/10.1093/jmcb/mjy049/5106402" target="_blank" >https://academic.oup.com/jmcb/advance-article/doi/10.1093/jmcb/mjy049/5106402</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jmcb/mjy049" target="_blank" >10.1093/jmcb/mjy049</a>
Alternative languages
Result language
angličtina
Original language name
A single synonymous mutation determines the phosphorylation and stability of the nascent protein
Original language description
p53 is an intrinsically disordered protein with a large number of post-translational modifications and interacting partners. The hierarchical order and subcellular location of these events are still poorly understood. The activation of p53 during the DNA damage response (DDR) requires a switch in the activity of the E3 ubiquitin ligase MDM2 from a negative to a positive regulator of p53. This is mediated by the ATM kinase that regulates the binding of MDM2 to the p53 mRNA facilitating an increase in p53 synthesis. Here we show that the binding of MDM2 to the p53 mRNA brings ATM to the p53 polysome where it phosphorylates the nascent p53 at serine 15 and prevents MDM2-mediated degradation of p53. A single synonymous mutation in p53 codon 22 (L22L) prevents the phosphorylation of the nascent p53 protein and the stabilization of p53 following genotoxic stress. The ATM trafficking from the nucleus to the p53 polysome is mediated by MDM2 and requires its interaction with the ribosomal proteins RPL5 and RPL11. These results show how the ATM kinase phosphorylates the p53 protein while it is being synthesized and offer a novel mechanism whereby a single synonymous mutation controls the stability and activity of the encoded protein.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of molecular cell biology
ISSN
1674-2788
e-ISSN
—
Volume of the periodical
11
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
"187–199"
UT code for WoS article
000467903600002
EID of the result in the Scopus database
2-s2.0-85067278382