All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

A single synonymous mutation determines the phosphorylation and stability of the nascent protein

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F19%3A00078030" target="_blank" >RIV/00209805:_____/19:00078030 - isvavai.cz</a>

  • Result on the web

    <a href="https://academic.oup.com/jmcb/advance-article/doi/10.1093/jmcb/mjy049/5106402" target="_blank" >https://academic.oup.com/jmcb/advance-article/doi/10.1093/jmcb/mjy049/5106402</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/jmcb/mjy049" target="_blank" >10.1093/jmcb/mjy049</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A single synonymous mutation determines the phosphorylation and stability of the nascent protein

  • Original language description

    p53 is an intrinsically disordered protein with a large number of post-translational modifications and interacting partners. The hierarchical order and subcellular location of these events are still poorly understood. The activation of p53 during the DNA damage response (DDR) requires a switch in the activity of the E3 ubiquitin ligase MDM2 from a negative to a positive regulator of p53. This is mediated by the ATM kinase that regulates the binding of MDM2 to the p53 mRNA facilitating an increase in p53 synthesis. Here we show that the binding of MDM2 to the p53 mRNA brings ATM to the p53 polysome where it phosphorylates the nascent p53 at serine 15 and prevents MDM2-mediated degradation of p53. A single synonymous mutation in p53 codon 22 (L22L) prevents the phosphorylation of the nascent p53 protein and the stabilization of p53 following genotoxic stress. The ATM trafficking from the nucleus to the p53 polysome is mediated by MDM2 and requires its interaction with the ribosomal proteins RPL5 and RPL11. These results show how the ATM kinase phosphorylates the p53 protein while it is being synthesized and offer a novel mechanism whereby a single synonymous mutation controls the stability and activity of the encoded protein.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of molecular cell biology

  • ISSN

    1674-2788

  • e-ISSN

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    13

  • Pages from-to

    "187–199"

  • UT code for WoS article

    000467903600002

  • EID of the result in the Scopus database

    2-s2.0-85067278382